TABLE OF CONTENTS
Methods of Semen Collection in Bulls
There are four methods of semen collection in bulls: the vaginal method, massage technique, artificial vagina method, and electroejaculation method.
Several methods of semen collection in bulls have been developed for the collection of semen. The methods of collecting semen have undergone several changes and mass changes have been made towards improving quality and quantity of the frozen semen.
Different semen collection methods in bulls are:
- Vaginal method
- Massage technique
- Artificial vagina method
- Electro ejaculation method
1. Vaginal Method
The simplest and earliest method of semen collection was from vagina.
In this method, the bull is allowed to mount the cow in or out of heat naturally and the semen is collected from vagina by means of a spoon or syringe with a long nozzle. This technique is unsatisfactory because selectively small volume of semen is mixed with large volume of vaginal mucus.
To avoid this female out of heat can be used. But this method requires the cow should be healthy and free of diseases.
2. Massage Technique
Massage Technique, here semen is collected by massaging the ampullae and seminal vesicle through the rectum.
This method was reported as early as 1925 by Case and later well described by Miller and Evans (1934).
3. Artificial Vagina (AV) Method
Artificial Vagina(AV) method of semen collection has replaced all the old, cumbersome and unreliable procedures.

The AV provides the most satisfactory method of collecting semen from bulls.
4. Electro Ejaculation Method
Electrical stimulation of ampullae and seminal vesicle is also a method of collecting semen from bulls.

Many instruments has been devised the collection of semen through electro ejaculation.