Summary of the Signs of Infertility
The following summary of the signs of infertility briefly describes a procedure for investigating an infertile animal on the basis of clinical history, signs and examination with an indication of possible diagnosis.

No Observed Estrus
- Palpate per rectum for pregnancy.
- If there is any doubt re-examine at a latter date.
- If not pregnant palpate ovaries.
Ovaries
- If the ovaries are small, narrow and functionless in a heifer, then this may be due to
- Delayed puberty
- Ovarian hypoplasia
- Possibly freemartinism
- Flattered, smooth, small and inactive ovaries.
- anestrus
- Presence of CL
- Pregnancy
- Non detected estrus
- Silent estrus
- Small active ovaries
- Proestrus, estrus or metestrus.
- Re – examination after 10 days for confirmation of CL.
- Large ovaries
- Ovarian cysts (luteal).
- One or both ovaries enlarged containing fluid filled thick walled structures.
- Persistant CL
- A persistent CL may be associated with pyometra, chronic endometritis, mummified fetus etc.,
- Careful examination of uterus helps to diagnose the case.
Prolonged Interestrus Interval
- Non detected estrus
- If the interval between successive heats is approximately twice the normal interval i.e., 38 – 48 days then this indicates one estrus has been missed.
- Embryonic or fetal death.
- The interval between successive heats will not be in the multiple of 21 (eg.) 30 days. In individual cow it is not of much significance. If a number of animals are involved especially if natural service is used, specific pathogens should be eliminated.
Regular Return to Estrus (Repeat Breeder)
- Poor quality semen
- Check the semen quality and general fertility
- Incorrect timing of AI or service
- Unlikely to occur repeatedly
- Nutritional deficiency of excess
- Check diet
- Anatomical defects
- Check for adhesions
- Occulded uterine tubes
- Carefully palpate or use PSP dye test
- Endometritis
- Clinical signs
- Delayed ovulation / anovulation
- Long estrus
- Luteal deficiency
Short Inter-estrous Interval
- Enlarged ovaries.
- Cystic ovaries / follicular cyst.
- AI at wrong time.
- Endometritis.
Correct Timing of Artificial Insemination
- The estrus is short in the cow 12-24 hours.
- Ovulation occurs 10-12 hours after the end of the estrus.
- Best conception rates occur if inseminated in the middle to the end of the standing estrus, i.e., 13-18 hours before evulation.
- Cows may conceive to AI at the beginning of estrus or 36 hours after the end of estrus but the conception rates are reduced.
- The correct timing of the AI is thus dependent upon true, accurate and early identification of estrus.
- A cow that is first seen in estrus in the afternoon or early morning is inseminated in the morning.
- A cow seen in estrus later in the morning, is inseminated in the afternoon of the same day.